getDeadLocations {qfa}R Documentation

Find dead cultures in SGA plates (1536 format), and report their location in spotted plates (384 format).

Description

Given Colonyzer quantifications of SGA plates in 1536 format, locate dead cultures and report the 384-format locations of those cultures for stripping.

Usage

getDeadLocations(SGAFile,SGAExpt,CutoffFrac=0.0025)

Arguments

SGAFile

The Colonyzer output for the final SGA plates. These are 1536 format plates, and the Colonyzer file should be a .dat tab delimited text file.

SGAExpt

Experiment description file for the SGA, linking barcode with plate number.

CutoffFrac

Optional argument for specifying the minimum value for Growth (normalised IOD) corresponding to detection of cells on 1536 plate.

Value

A data frame containing columns ROW384, COLUMN384, REP384 (repeat or quadrant identifier) and PLATE which will be useful for stripping.


[Package qfa version 0.0-45 Index]